WebBacterial directional TOPO® cloning vector, for tagging a protein N-terminally with His-Patch thioredoxin and C-terminally with V5 and 6xHis. ... Explore Over 2.7k Plasmids: TOPO … Web13 rows · Welcome to Vector Database! Vector database is a digital collection of vector backbones ...
8/GW/TOPO TA Cloning Kit - Thermo Fisher Scientific
WebAbstract. We evaluated the cloning efficiency of different size PCR products into three T-vector cloning systems. The pGEM®-T and pGEM®-T Easy Vector Systems gave a high number of recombinants across a broad range of insert sizes (0.5–3kb) while the TOPO TA Cloning® system worked well for inserts less than 1kb, but showed a striking decrease in … Webg and Gateway® technologies to facilitate 5-minute, 1-step cloning of Taq polymerase- amplified PCR products into a plasmid vector with greater than 95% efficiency. As is the case with other pCR ™ vectors (e.g. pCR 2.1-TOPO®), … fabletics sale items
Topographic map - Wikipedia
WebThe sequence above represents the pCR®2.1-TOPO vector with a PCR product inserted by TA Cloning®. The arrow ( ) indicates the start of transcription for the T7 RNA polymerase. T7 Promoter M13 Forward (-20) Primer M13 Forward (-40) Primer NotIXhoINsiIXbaIApaI +1 pCR®2.1-TOPO 3.9 kb M13 Reverse Primer lacZ α ATG Hind IIIKpnISacIBamH ISpeI WebJun 21, 2016 · Topo Map Vector Data. The Topo Map Vector Data products are one specific type of staged product available from TNM. They are intended for a variety of GIS and cartographic applications. These products are staged at a 7.5-minute footprint to correspond with the US Topo map 7.5-minute extent. They contain feature classes from … WebI am getting vector alone colonies and no insert with topo cloning. Can anywone please suggest me what could the possible mistake. My vector size is 3.9kb and insert size is … fabletics seamless high waisted legging